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VOL. 8, ISSUE 2 (2023)
In vitro propagation of threatened terrestrial orchids Phaius tankervillieae (L’Her) blume and Geodorum densiflorum (Lam.) schltr. via seed pod culture
Authors
Indrajit Kalita, Anwesha Borah, Chandra Barooah
Abstract
The orchids belongs to orchidaceae family is the second largest and
most diverse group of plants and it is widespread all over the globe, except
for in Polar Regions. Phaius tankervillieae commonly known as Nun’s hood
Orchid is one of the most widely valued ornamental orchids in the world. Geodorum
densiflorum is one of the floriculturally and medicinally important ground
orchids. Orchid’s seeds are small, numerous and non-endospermic. Germination
rate is very slow and some needs fungal association for germination. Tissue
culture has become the standard method of propagation for the conservation of
orchids. In the present experiment, we have analysed the effect of various
concentration of hormones in seed germination and protocorm formation of both
species with MS media. The mature capsule of both the species were used as
explant for present study. The seed germination rate of Phaius
tankervillieae and Geodorum densiflorum were found to be variable in
hormone free MS medium and MS medium supplemented with various concentration of
hormones. Quickest germination of Phaius tankervillieae seed was
observed on MS basal medium supplemented with 1mg/L kinetin + 1mg/L NAA which
was also found to be the best for fastest protocorm formation. However MS
medium when supplemented with 1.5mg/L kinetin + 1mg/L NAA produced maximum
number of shoots per culture and maximum number of roots with shoots per
culture. Fastest germination of Geodorum densiflorum seeds was observed
on MS basal medium supplemented with 2mg/L NAA + 2mg/L BAP and 2.5mg/L BAP+
0.1% AC which was also found to be the best for quickest protocorm formation.
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Pages:95-102
How to cite this article:
Indrajit Kalita, Anwesha Borah, Chandra Barooah "<i>In vitro </i>propagation of threatened terrestrial orchids <i>Phaius tankervillieae </i>(L’Her) blume and <i>Geodorum densiflorum </i>(Lam.) schltr. via seed pod culture". National Journal of Multidisciplinary Research and Development, Vol 8, Issue 2, 2023, Pages 95-102
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