There has been lots of development on biodiesel in different countries in the latest time and important things have picked up for it to produce it simply. In this investigation Jatropha seed oil (non-edible) is used and it is mixed with nano particle to find out its suitability for use as petro-diesel fuel. Investigation has been done to find out the different properties of blended fuel. Biodiesel was prepared from jatropha oil by transesterification process with ethanol by using NaOH as catalyst. The morphology of the nanoparticles are determined by Scanning Electron Microscope and the crystalline phase of nanoparticles are determined by X-ray Diffraction. The fuel obtained from experiment could be a major step towards the creation of an eco-friendly transportation fuel.